Arildo José Braz de Oliveira
Universidade Estadual de Maringá, Brazil
Title: Determination of the degree of polymerization of inulin using different analytical methodologies
Biography
Biography: Arildo José Braz de Oliveira
Abstract
This study discusses and shows as to determine the degree of polymerization (DP) of inulin, exemplified by samples obtained from roots of Brazilian medicinal plants as Stevia rebaudiana and Pfaffia glomerata, using different analytical methodologies as colorimetric methods, gel permeation chromatography coupled to multiangle laser light scattering and refractive index detectors (GPC/MALLS), nuclear magnetic resonance (NMR), gas chromatography coupled mass spectrometry (GC/MS), electron spray ionization mass spectrometry (ESI/MS) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS). It will be shown that these techniques and others can be used together to give complementary information thus providing a more accurate estimate of the overall DP of the inulin like molecules.